Comparison of five PCR methods for detection of Helicobacter pylori DNA in gastric tissues.

نویسندگان

  • J J Lu
  • C L Perng
  • R Y Shyu
  • C H Chen
  • Q Lou
  • S K Chong
  • C H Lee
چکیده

Five different PCR methods for the detection of Helicobacter pylori were evaluated. The results of this study indicate that of the five PCR methods examined, the ureC (glmM) gene PCR is the most sensitive and specific for the detection of H. pylori in gastric biopsy specimens.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Analysis of KRAS gene mutation associated with Helicobacter pylori infection in patients with gastric cancer

Objective(s): KRAS proto-oncogene mutation can be considered a diagnostic factor for treating various malignancies. Helicobacter pylori infection, a risk factor for stomach cancer, may cause DNA damage and genetic changes. The aim of the current study was to assess the association of gastric cancer and KRAS mutation, demographic factors, and H. pylori infection.<...

متن کامل

Comparison PCR Method and Rapid Urease Test to Detect Helicobacter Pylori in the Gastric Biopsy Tissue Samples

Background & Objective: Helicobacter pylori has been recognized as a major risk factor in gastric and duodenum ulcers and gastric cancer. Some laboratory tests, such as culture, are not entirely satisfying. The aim of this study is to compare RUT with PCR in identifying H. pylori in the gastric biopsy tissue samples.Materials & Methods: First, the standard H.pylori sample (N:oC30) was provided....

متن کامل

Detection of Helicobacter DNA in Bile Samples of Patients with Biliary Diseases Living in South of Iran

Background: It has been reported that several species of Helicobacter colonize the biliary tract of animals and human, but their participation in hepatobiliary diseases are not established. This study is undertaken to determine if Helicobacter genus members, especially with regard to H. pylori, could be detected in the bile and gallbladder diseases. Methods: Thirty-eight gallbladder tissues and...

متن کامل

Simultaneous Detection of Helicobacter Genus and Helicobacter pylori Species using a Multiplex PCR Method

In order to improve simultaneous detection and identification of Helicobacter genus in general and Helicobacter pylori specifically and reduce the number of amplifications needed, we established a multiplex PCR. In this study, two pairs of primers: Hcom1 and Hcom2 specific for Helicobacter genus, Hicd1 and Hicd2 specific for Helicobacter pylori species were used. To determine the sensitivity of...

متن کامل

Detection of Helicobacter pylori from Gastric Biopsy Samples by Culture, Polymerase Chain Reaction and Histopathological Methods in Eastern Turkey

The aims of this study were to detect Helicobacter pylori (H. pylori) by culture, polymerase chain reaction (PCR) and histopathological methods, to determine the prevalence of active H. pylori infection in Elazig Province, East of Turkey and to evaluate the relationship between H. pylori infection and sex. Antrum and corpus samples of 184 Turkish patients (85 male and 99 female, age range 17 to...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Journal of clinical microbiology

دوره 37 3  شماره 

صفحات  -

تاریخ انتشار 1999